This test uses transfected hep 2 cells human epithelial, which allow specific identification of autoantibodies to ssaro antigen. The detection of ana in human serum is an important screening tool for connective tissue diseases, and iif is the reference method for ana testing 1. Early experiences in mitotic cells recognition on hep 2 slides conference paper pdf available in proceedings of the ieee symposium on computerbased medical systems. Limitations of antinuclear antibody tests hep2 are. The immunoconcepts hep 2000 is a indirect enzyme add antimmunoglobin antibody test for the semiquantitative detection of ana in human serum. Hep2go is an online home exercise program tool for rehabilitation professionals. Human throat epidermis hepcancer cell line was pur 2 chased from cell bank of chinese academy of sciences.
In this study, we investigated the antiproliferative activity and the effects of rljrgd4 on the migration and invasion of hep 2 cells. Hepatitis b virus induces g1 phase arrest by regulating. Cells that are difficult to detach may be placed at 37c to facilitate dispersal. Original article construction of human hepg 2 cells infected by lentivirus carrying green fluorescent protein gene wei zang 1, lihua xie2, baohua zhu, dawei cui1 1department of hepatobiliary surgery, jilin central hospital, jilin 2000, jilin province, china. Mining knowledge for hep2 cell image classification petra perner. Theyweregrownoncoverslips,washedwithphosphatebufferedsaline, andfixedin acetoneat200cfor5 min. An automated pattern recognition system for classifying. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. Antinuclear antibody screening using hep2 cells ncbi.
To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells should be passaged when a 7080% confluency is reached. Pdf cell lines represent an invaluable resource in modern science including basic and translational cancer research. To confirm our findings, we used another two cancer cell lineshne2 and a549 cells to investigate the mtdna copy number variation after ddp or dox treatment. Hep 2 is a cell line derived from hela they were are contamination in an originally hepatic cell line see atcc for details and so are a cervix carcinoma cell line, most useful, because they have a rather big nucleus. Clinical relevance and frequency of cytoplasmic and nuclear dense. Icap was initiated as a workshop aiming to thoroughly discuss and to promote consensus regarding the richness in nuances of morphological patterns observed in the indirect immunofluorescence assay on hep 2 cells. Early experiences in mitotic cells recognition on hep2 slides. In jra patients, the speckled pattern occurred most commonly 72%. Applic on n hepg2 labeling and cell growth protocol for.
Reducedmtdnacopynumberincreasesthesensitivity of tumor. Typical patterns of ana using hep2 cells atlas of antinuclear. For physical therapists, occupational therapists, athletic trainers, and other qualified rehab professionals. In addition to weakened or killed disease antigens viruses or bacteria, vaccines contain very small amounts of other. When interpreting the patient sample, both resting and mitotic cells should be evaluated. Our results indicated that either rljrgd3 or rljrgd4 can inhibit cell proliferation via inducing apoptosis in hep 2 cells. Inhibition of hepatitis b virus production by boehmeria. In parts of china and taiwan, 80% of hepatocellular carcinoma is due to hepatitis b.
Hep2go online home exercise program rehab physical. The saffold viruspenang 2b and 3c proteins, but not the l. Consistent with the hep 2 cells, qpcr data showed that the mtdna copy number of early apoptotic tumor cells was also significantly. The fluorescence patterns of the two substrates can be compared and results verified reciprocally. This cell line was originally propagated from a humanlaryngeal carcinoma. In contrast, the it1 cell line possesses ideal requirements by being originated from nonmalignant cells and also by being a macrophage that plays a pivotal role in the immune response mechanism. Origin of hep 2 cells used for culture of chlamydiae. Edgbaston birmingham b15 2tt united kingdom main switchboard. Get a printable copy pdf file of the complete article 403k, or click on a page image below to browse page by page. Media in category hep2 cells the following 4 files are in this category, out of 4 total. They allow for recognition of over 30 different nuclear and cytoplasmic patterns.
Scribd is the worlds largest social reading and publishing site. Instead, they were funneled through an amherst town highway garage and received a hepatitis booster shot by sticking their arms out of car windows. The fluorescence test for ana is performed by incubating the patients serum with a fixed monolayer of human larynx epithelioma cancer hep2 cell lines. Hep g2 is an immortal cell line which was derived from the liver tissue of a 15yearold african american adolescent boy with a welldifferentiated hepatocellular carcinoma.
Exposure of hep2 cells to stress conditions influences. Moreover, analysis of primate liver alongside hep 2 cells provides additional security in the evaluation of ana iift results. Use of the hep2 cell substrate in the detection of. Classification of hep2 cells using fluorescent image. Fourteen patients 8 with pauciarticular onset and 6 with polyarticular onset had iridocyclitis. The hep2 cell, a native protein array with hundreds of antigens, provides an ideal substrate for the detection of ana 1. Hep 2 cells in the nude mouse model remains unclear. Hepatitis b surface antigen hbsag, hepatitis b virus e antigen hbeag, and hbv dna were measured by using elisa and realtime pcr, respectively. However, the fact that the hep 2 cell line originated from laryngeal carcinoma cell was the original sin that hep 2 could not escape from.
Different types of ana give rise to characteristic staining patterns on the hep2 cells, depending on the cellular. This was more significant in the inoculated cells with isolates 34, 37 and 174 kruskalwallis, p hep 2 cells showed a gradual ab figure 1 lscm optical sections showing internalization of u. The results suggest that stressrelated ana are present in a fraction of patients diagnosed with a connective tissue disease ctd, such as sle or. The cells in this culture were heterogeneous with regard to the expression of viral antigens, ranging from high to undetectable levels.
Antimicrobial susceptibility, biofilm production and. Clinical relevance of hep2 indirect immunofluorescent patterns. Original article rljrgd4, an rgd toxin protein from. Origin of hep2 cells used for culture of chlamydiae. The fimbriae or pili promote the adherence of the bacterium to receptors on the surface of the host cell, and the type iv fimbria. Recently, computeraided diagnosis of autoimmune diseases by the iif hep 2 cell classi. Mining knowledge for hep2 cell image classification. Pdf download atlas of antinuclear antibodies mbl medical. Feature importance for human epithelial hep2 cell image. The use of hep 2 cells provided a sensitive substrate for detecting ana in jra. Mbl,diagnostic reagents,atlas of antinuclear antibodies. A total of 1,385 people received the hepatitis a vaccine in amherst on sept. Pdf feature importance for human epithelial hep2 cell image.
Finally, remarks of the hep2 cell pattern recognition are presented in section vi. Pdf the american college of rheumatology position statement on ana testing stipulates the use of iif as the gold standard method for ana. The cells that are considered in this application for an automated image analysis are hep 2 cells which are used for the identification of antinuclear autoantibodies ana. In this study, we screened for the viral proteins of safv causing apoptosis in the host cells and further. The term antinuclear antibody ana describes a variety of autoantibodies that react with constituents of cell nuclei including dna, proteins and ribonucleoproteins 1,2. Antimicrobial susceptibility, biofilm production and adhesion to hep 2 cells of. The term antinuclear antibody ana describes a variety of autoantibodies that. Ac anticell ama antimitochondrial antibody crest limited cutaneous form of ssc referred to as crest syndrome and contains these five symptoms. Hep 2 cells allow for recognition of over 30 different nuclear and cytoplasmic patterns, which are given by. Hep2 cells are used for the identification of antinuclear autoantibodies. Hep2 atcc ccl23 homo sapiens hela contaminant carcinoma.
After having done this, we went into a structured interviewing process asking for. As a result, when antissaro antibodies are the only anas present, one of two distinct patterns may be seen. Contribute to petters hep 2 development by creating an account on github. Causes hepatitis b and c the two most important etiological factors contributing to hepatocelluar carcinoma are hepatitis b and hepatitis c figure 5. Ultrastructural analysis of cells revealed that, compared to hepg2 cell, the hepg2. Original article construction of human hepg2 cells. The remainder are a mixture of the four phases of mitosis. Pdf antinuclear antibody screening using hep2 cells. Antinuclear antibody screening using hep2 cells protocol. This paper presents an experimental analysis of five different categories of feature sets with four.
Cultures of hep2 cells persistently infected by human. Hepg2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15yearold caucasian male who had a welldifferentiated hepatocellular carcinoma, which is the fifth mostcommon cancer worldwide. Pdf indirect immunofluorescence iif microscopy imaging of human epithelial hep2 cells is a popular method for diagnosing. The hepg2 cell line is commonly used in drug metabolism and hepatoxicity studies. The pattern recognition step is an important one as. Cell culture cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells e. Virus inclusions containing virus particles were visible in the cytoplasm of hepg2. Hep 2 cell image classification is an important and relatively unexplored area of research.
Human epithelial type 2 hep2 cells, considered to originate from a human laryngeal carcinoma, allow recognition of over 30 different nuclear and cytoplasmic patterns that are given by upwards of 50 different autoantibodies are associated with various autoimmune conditions. So, if you need liver cells, hep 2 are a bad choice. Hep 2 cells that survived a lytic infection with human respiratory syncytial virus hrsv were grown to obtain a persistently infected culture that produced relatively high amounts of virus 10 6 10 7 pfuml for more than twenty passages. Vaccine excipient summary excipients included in u. Currently, iif using a cell line of human larynx epithelioma hep2 human epithelioma type 2 ccl 23atcc clone virtually replaced the imprint of rodent cells. Humanepitheliomacells hep 2 were obtained from antibodies inc. Viral dna replication and rna expression were determined by using southern and northern blot.
Rpmi1640, dmem, fetal bovine serum fbs were purchased from hyclone. Research article open access apoptosis in hep2 cells. Vast majority of these patterns will appear on this site. However, only about 10% 20% of the cells demonstrate this hyperexpression.
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